1. Angiogenesis

• Tube assay
• Matrigel
• Ring assay (no of rings)
• ii. tube assay (length of the tube)
• iii. Collateral (no and length of the tube)
• In ovo assay (no, length, no of capillaries)
• In vivo assay
  • Cotton plug assay (rat)
  • Matrigel assay (mouse)
  • Matrigel-Sponge assay (rat)
• Scaffold (Growing blood vessels in 3D scaffolds)
  • i.Matrigel
  • Sodium alginate
  • Agar-serum

2. Permeability

• Single cell trypan blue
• Monolayer trypan blue/Albumin FITC

3. Migration

• Wound healing
• Single cell migration
• Single Transwell chamber
  • Chemotaxis
  • Chemokinesis
• Modified Boyden chamber (48 wells)
  • Chemotaxis
• Chemokinesis

4. Nitric oxide estimation (cells/tissue/organ/animals)

• DAF
  • Live cell Imaging
  • Live cell fluorimetry

• GRIESS assay
• Ultra-sensitive NO electrode
• EPR (Spin trapping – Electron Paramagnetic Resonance spectroscopy)

5. Reactive Oxygen species and Oxidative stress assays

•   Superoxide assay
  • Spin trapping (Electron Paramagnetic Resonance spectroscopy)
  • NBT assay
  • Pyrogallol (SOD activity) assay
• TBA (lipid peroxidation)
• Peroxynitrate - flurimetric assay with 1,2, 3 dihydrorhodamine
• Glutathione assay

6. Co-culture model

• (pericyte-endothelial cells)

7. 3D gel contraction assay

• Contractile profile of contractile cells

8. Hypoxia

• Normoxic hypoxia in hypoxia chamber
• Real time imaging under hypoxia – Real time imaging hypoxia chamber

9. Microgravity

• Treatment of cells and tissues under microgravity – clinostat (microgravity machine)
• Hind limb suspension animal model – under standardization

10. Isolated organ/tissue physiology work

• Isolated aorta ring contraction assay – kymograph
• Isolated skeletal muscle contraction assay – kymograph