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Recent advances in the
technology and development of physical methods enable one to probe biomolecular
processes in real-time at single molecule level. These led to the study of biophysical
properties of single molecule DNA, folding & unfolding of proteins, transcription
and translation activity in real-time, single molecule DNA-protein interactions,
enzymatic kinetics etc. Such studies involve nanoscale manipulation of biomolecules.
The challenge in doing science at this level is to deal with the length scale
of few nanometers and an energy scale of few KBT (~10-21J).
At these scales, viscous forces dominate both animate and inanimate objects
which are compounded with the Brownian noise arising out of fluctuations in
KBT. Our lab is focused on the development of such nanoscale manipulation
and detection methods using optical tweezers & fluorescence microscopy and
their application in biology broadly in the domain of spatio-temporal characterization
of DNA-protein interaction.
We are currently working
on the following projects:
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